Switch-like surface binding of competing multivalent particles

Controlling Multivalent Binding through Surface Chemistry: Model Study on Streptavidin

Although multivalent binding to surfaces is an important tool in nanotechnology, quantitative information about the residual valency and orientation of surface-bound molecules is missing. To address these questions, we study streptavidin (SAv) binding to commonly used biotinylated surfaces such as supported lipid bilayers (SLBs) and self-assembled monolayers (SAMs). Stability and kinetics of SA...

Binding Effects in Multivalent Gibbs-Donnan Equilibrium

The classical Gibbs-Donnan equilibrium describes excess osmotic pressure associated with confined colloidal charges embedded in an electrolyte solution. In this work, we extend this approach to describe the influence of multivalent ion binding on the equilibrium force acting on a charged rod translocating between two compartments, thereby mimicking ionic effects on force balance during in vitro...

Binding of Multivalent Ligands to Cells: E ects of Cell and Receptor Density Condensed title: Binding of Multivalent Ligands (Keywords: Multivalent Ligands, Crosslinking

We study the equilibrium binding properties of multivalent ligands and mixtures of ligands of diierent valence to cell surface receptors. We examine the eeects of changing cell density or number of receptors per cell, i.e., receptor concentration, over a wide but physiologically relevant range. Qualitatively diierent behaviour arises in the excess receptor regime, where the volume concentration...

Bridging lectin binding sites by multivalent carbohydrates.

Carbohydrate-protein interactions are involved in a multitude of biological recognition processes. Since individual protein-carbohydrate interactions are usually weak, multivalency is often required to achieve biologically relevant binding affinities and selectivities. Among the possible mechanisms responsible for binding enhancement by multivalency, the simultaneous attachment of a multivalent...

Ig Class Switch Recombination via Papillomavirus - Like Particles Directly B Lymphocyte